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Unit 4 - perls' prussian blue

memorize.ai (lvl 286)

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T/F - dye is not used

Front

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Perl

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Last updated

1 year ago

Date created

Mar 1, 2020

Cards (17)

Section 1

(17 cards)

T/F - dye is not used

Front

False, a counterstain such as eosin or safranine is used

Back

Slides are placed in equal amounts of

Front

2% potassium ferrocyanide and 2% HCl and heated for 20 min at 60C

Back

What causes an increase in iron deposits?

Front

increased rbc destruction

Back

T/F - chemical reaction is used to produce colour

Front

true

Back

How to correct for faded colour?

Front

restore colour by oxidizing ferrous to ferric with hydrogen peroxide

Back

If counterstained for too long, iron shows up what colour

Front

black

Back

Which counterstains can be used for perls or turnbulls

Front

eosin (acid dye) or safranine (basic dye)

Back

Glass ware should be soaked in

Front

10% nitric acid (several hours) to remove organic matter

Back

What does turnbull's blue reaction demonstrate?

Front

ferrous iron in tissues

Back

What type of mounting media should be used

Front

synthetic neutral mounting media (saturated polystyrenes)

Back

Reason for performing PPB?

Front

ferric iron demonstration, looking for abnormal iron deposits

Back

What precautions should be made in regards to equipment used?

Front

avoid metal, use plastic, use acid washed glassware for HCl and kferrocyanide solution containers

Back

Why does hemoglobin and formalin pigment have no reaction?

Front

hemoglobin is ferrous iron so no reaction, iron is also very strongly bonded in hgb

Back

Large amounts of iron found in

Front

liver, spleen, lymph nodes, bone marrow with rbc destruction, increased iron intake, hemorrhages, cirrhosis

Back

T/F - iron is not usually stored in tissue in ferrous form as it is very toxic

Front

true

Back

Small amounts of iron found in

Front

spleen and bone marrow

Back

What type of fixative should be used

Front

10% NBF

Back