Speciation only occurs when there is an advantageous new trait that allows it to become a new species, in sexually reproducing species (reduced gene flow in sexual species, increased gene flow/genetic material in bacteria)
1. separate populations allows for independent evolvement (isolation)
2. sexual selection causes the divergence of new traits (can lead to polyploidy/mutations)
3. reproductive isolation (hybridization, two species that mate and can form a hybrid that is either selected against and die or not sterile)